에탄올이 Streptococcus mutans의 atpB 유전자 발현 및 양성자 투과성에 미치는 영향
Ethanol changes atpB gene expression and proton permeability in Streptococcus mutans
조철민, 박용진, 이새아, 김진범, 강정숙,
소속 상세정보
조철민 ( Cho Chul-Min ) - 부산대학교 치의학전문대학원 구강생화학교실
박용진 ( Park Yong-Jin ) - 부산대학교 치의학전문대학원 구강생화학교실
이새아 ( Lee Sae-A ) - 부산대학교 치의학전문대학원 구강생화학교실
김진범 ( Kim Jin-Bom ) - 부산대학교 치의학전문대학원 예방과사회치의학교실
강정숙 ( Kang Jung-Sook ) - 부산대학교 치의학전문대학원 구강생화학교실
Abstract
Objectives: As a first step to study the anticaries effect of ethanol alone, we investigated the effects of ethanol on the expression levels of the atpB gene and proton permeability of Streptococcus mutans in suspension cultures.
Methods: S. mutans UA159 was grown in brain heart infusion medium at either pH 4.8 or 6.8. The total extracted RNA was reverse-transcribed into cDNA using a SuperscriptTM First-Strand Synthesis System. The resulting cDNA and negative controls were amplified by ABI PRISM 7700 real-time PCR system with SYBR Green PCR Master Mix. For proton flux assay, bacterial suspensions were titrated to pH 4.6 with 0.5 M HCl, and then additional 0.5 M HCl was added to decrease the pH values by approximately 0.4 units. The subsequent increase in pH was monitored using a glass electrode. Ten percent (v/v) butanol was added to the suspensions at 80 min to disrupt the cell membrane.
Results: In a concentration-dependent manner, ethanol alone not only decreased the growth rate of S. mutans and the expression of the atpB gene but also increased the proton permeability at both pH 4.8 and 6.8.
Conclusions: These findings suggest that ethanol has the potential for an anticaries ingredient. We believe that ethanol may be used together with fluoride and/or other cariostatic agents in order to develop better anticaries toothpastes and/or mouthrinses.
키워드
Ethanol; atpB gene expression; Proton permeability; Streptococcus mutans
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